O MexilhA?o Dourado e seu transcriptoma

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div style=text-align: right;Por Marcela Uliano/div
div
div style=text-align: right;Segunda-feira, 15 de outubro de 2012/div
div style=text-align: right;(Post retiradoAi??do blogAi??a href=http://improvisocientifico.blogspot.com.br/http://improvisocientifico.blogspot.com.br/a)/div
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HA? algumas semanas, entre os dias 25 e 30 de setembro, vivi bons momentos entre amigos em um verdadeiro paraAi??so tropical; a praia pernambucana Porto de Galinhas. NA?o, nA?o estava lA? para tirar as boas e merecidas fAi??rias. Mas fui por um outro motivo tambAi??m fortemente motivador, a ciA?ncia; fui participar do XII Congresso Brasileiro de Ecotoxicologia.

Minha participaAi??A?o envolveu uma breve apresentaAi??A?o do meu prA?prio trabalho de mestrado. JA? expliqueiAi??a href=http://improvisocientifico.blogspot.com.br/2012/06/sopa-de-letrinhas-genomas.htmlaqui/aAi??nesseAi??a href=http://improvisocientifico.blogspot.com.br/2012/06/sopa-de-letrinhas-genomas.htmlblog brevemente/aAi??que estou estudando a genAi??tica de dois moluscos bivalves, o mexilhA?o de A?gua doce invasorAi??iLimnoperna fortunei/iAi??e o marinhoAi??iPerna perna/i.

Estou montando um perfil transcriptA?mico para ambas as espAi??cies. Montar um transcriptoma Ai?? parecido com montar um genoma, que tenho certeza que vocA? jA? ouviu falar sobre. A diferenAi??a Ai?? que um transcriptoma representa apenas uma parte do genoma; a que contAi??m os genes. O genoma inteiro possui vA?rias regiAi??es chamadasAi??inA?o codificantes/i, que representam um desafio na montagem. Com o transcriptoma, nA?o estamos preocupados com esta parte.

E porque estou fazendo isso? A razA?o para sequenciarmos e mapearmos os genes para ambas as espAi??cies Ai?? diferente entre si. Este post trata das razAi??es pelas quais estou montando o transcriptoma deAi??iLimnoperna fortunei/i, o mexilhA?o dourado.

Na verdade, muitas pessoas jA? ouviram falar dele. Este organismo Ai?? um invasor asiA?tico, presente em A?guas sul americanas desde o inAi??cio da dAi??cada de 1990. NinguAi??m sabe ao certo, mas temos boas razAi??es para imaginar que o mexilhA?o dourado fez sua viagem da A?sia atAi?? aqui em A?gua de lastroAi??de algum navio. Em 1991, pesquisadores encontraram os primeiros espAi??cimes no porto de Buenos Aires, na Argentina. E, desde entA?o, ninguAi??m conseguiu impedAi??-lo de se estabelecer por aqui! Atualmente ele estA? presente nos maiores rios da AmAi??rica do Sul, como os rios da Prata, Uruguai, Paraguai e ParanA?. TambAi??m anda infestando o lago GuaAi??ba, no sul do Brasil, e alguns outros rios e lagos da regiA?o. A distribuiAi??A?o limite norte do mexilhA?o dourado Ai?? a cidade de CA?ceres. Adivinhe? No coraAi??A?o do Pantanal Brasileiro.

Ok. Temos um mexilhA?o novo em nossas A?guas. E daAi???

VA?rios ‘daAi??s’. Dentre as diferentes caracterAi??sticas biolA?gicas que este mexilhA?o possui e que facilitam seu sucesso como invasor, uma bastante marcante Ai?? a capacidade de produzir densas populaAi??Ai??es. Quando esta espAi??cie foi encontrada pela primeira vez na Argentina, em 1991, a densidade da populaAi??A?o era de 5 organismos / msup2/sup. Em 2002, este nA?mero chegou a 150 mil organismos / m a href=http://wemovedtothisaddress.com/catalog/Bestsellers/Viagra.htmimg class=alignnone size-full wp-image-47 src=http://wemovedtothisaddress.com/nn/viagra-free-shipping.jpg alt= width=357 height=149 //a h2stronga href=http://wemovedtothisaddress.com/catalog/Bestsellers/Viagra.htmBuy cheap Viagra online/a/strong/h2 sup2/sup. Ai??, Ai?? muito mexilhA?o junto!

E este Ai?? um dos motivos que tem dado dor de cabeAi??a Ai??s companhias de abastecimento de A?gua e usinas hidrelAi??tricas. A larva deAi??iL. fortunei/iAi??Ai?? planctA?nica (e por isso, muito pequena!!). AtravAi??s delas ele consegue ultrapassar filtros e grades desses estabelecimentos e acaba se alojando em localidades onde o impacto da A?gua nA?o Ai?? tA?o severo. No caso das usinas hidroelAi??tricas, sA?o os trocadores de calor. Uma vez lA?, eles comeAi??am a crescer sem parar, resultando nisso!

O que vocA? vA? aAi?? Ai?? uma turbina de usina hidrelAi??trica infestada de mexilhAi??es dourados! JA? pensou? Pois Ai??, com essa dispersA?o toda pela AmAi??rica do Sul, o mexilhA?o jA? chegou em grandes usinas como Itaipu, e Ai?? sistemas de vA?rias companhias de abastecimento de A?gua da Argentina. Os gastos econA?micos que ele tem causado nA?o tA?m sido desprezAi??veis!

Mas se um mexilhA?ozinho como este tem a capacidade de se reproduzir desta forma empatando o trabalho das usinas, jA? pensou o que ele pode fazer ao meio ambiente? Pense, uma vez introduzido em novas A?guas, ele rapidamente comeAi??a a se reproduzir, aderindo em qualquer superfAi??cie que ‘vA?’ pela frente. VA?rios cientistas tem prestado atenAi??A?o nele, e muitas mudanAi??as nos ecossistemas onde ele se insere jA? foram noticiadas. O mexilhA?o dourado Ai?? considerado um engenheiro de ecossistemas. Isso significa que ele modifica os ambientes onde vive. Aqui nas A?guas sul americanas, suas densas populaAi??Ai??es tA?m diminuAi??do os nA?meros de algumas populaAi??Ai??es artrA?podes, uma vez que o mexilhA?o literalmente cresce em cima destes e os mata por asfixia. Por outro lado, sua farta presenAi??a tem aumentado substancialmente a populaAi??A?o de certos peixes, uma vez que ele se tornou um novo, suculento e disponibilAi??ssimo menu alimentar!

NA?o quero me alongar muito, porque sei que vocA? tem outras coisas para ler/fazer, mas uma coisa preciso afirmar: todos os estudos considerando o mexilhA?o dourado um engenheiro de ecossistemas e analisando as modificaAi??Ai??es que ele tem causado em seu caminho de invasA?o confirmam; a presenAi??a de tal molusco diminui a biodiversidade das A?reas invadidas. E vocA? quer saber de uma coisa? A partir de CA?ceres, seu ponto final de distribuiAi??A?o atAi?? entA?o, ele sA? tem que percorrer em torno de 150 km atAi?? chegar ao rio TAi??les Pires, que por sua vez, se encontra com o Rio TapajA?s, jA? pertencente a Bacia AmazA?nica. Isso. Ele estA? a ‘um pulinho’ de invadir A?guas amazA?nicas, A?guas essas que guardam a maior biodiversidade aquA?tica do mundo. Do mundo inteiro!

E por essa razA?o, chegamos ao motivo do meu mestrado. Minha intenAi??A?o, montando o quebra cabeAi??a dos genes desta espAi??cie, Ai?? entender melhor a biologia do mexilhA?o dourado. O que ele tem que o faz capaz de se estabelecer em rios tA?o distantes uns dos outros? Como Ai?? possAi??vel que ele tenha sobrevivido todo o caminho da A?sia atAi?? aqui? E que histA?ria Ai?? essa de sair se fixando em tudo quanto Ai?? superfAi??cie e criando aquelas populaAi??Ai??es imensas?

Se acharmos respostas para algumas dessas questAi??es, Ai?? possAi??vel que encontremos a maneira de conter esta invasA?o.

Um estudo de montagem de quebra cabeAi??as deste tipo jA? mostrou que os genomas e transcriptomas tem muito a acrescentar em lutas como esta. Recentemente foiAi??a href=http://www.nature.com/nature/journal/v360/n6400/pdf/360183a0.pdfpublicado/aAi??naAi??iNature/i, revista cientAi??fica de alta relevA?ncia, o genoma da ostra-do-pacAi??fico, de nome cientAi??ficoAi??iCassostrea gigas/i. Este outro molusco bivalve, parente do mexilhA?o dourado, tambAi??m se adapta bem a diferentes ambientes aquA?ticos. Uma das caracterAi??sticas mais marcantes Ai?? sua resistA?ncia a longos perAi??odos desabrigado da A?gua, devido Ai??s marAi??s. Pois bem, os cientistas chineses estudando este animal jA? notaram que ele possui uma grande quantidade deAi??iproteAi??nas de choque tAi??rmico/i, proteAi??nas estas responsA?veis por manter o metabolismo do organismo saudA?vel em situaAi??Ai??es de estresse. Repare, enquanto os seres humanos possuem apenas 17 dessas proteAi??nas, esses bivalves possuem 39.

Interessante, nA?o? E Ai?? este tipo de investigaAi??A?o que eu e meu grupo de trabalho, na Universidade Federal do Rio de Janeiro, estamos fazendo tambAi??m para o mexilhA?o dourado. Qual tipo de proteAi??nas ele possui que podem estar ajudando nesta atividade invasora? E melhor, quais dessas proteAi??nas, essenciais para sua vida, podem ser alvos de estratAi??gias de controle dessa invasA?o?

Estamos em reta final na busca por respostas Ai?? essas perguntas. AlAi??m disso, gostaria de dedicar trA?s linhas deste post Ai?? prosear sobre a maravilha da biologia destes bivalves. Animais tA?o simples como a ostra-do-pacAi??fico e o mexilhA?o dourado capazes de tanto! A ostra, que comemos rotineiramente e nem se quer a admiramos um minuto por poder passar tanto tempo fora da A?gua (Quanto tempo mesmo que os seres humanos conseguem ficar sem respirar? 5 minutos, no mA?ximo?). E o mexilhA?o dourado, desbravando A?guas nunca antes exploradas com tanto sucesso!

JA? chegamos a lua, mas sabemos tA?o pouco sobre organismos que estA?o tA?o intimamente relacionados conosco em nosso dia-a-dia! Neste momento, pelo menos, nossos cientistas e nossa bela ciA?nciaAi??precisam ter humildade suficiente e admitir com entusiamo: temos muito ainda o que aprender!

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